产品货号：
GS4493
中文名称：
重组人PDGF-BB
英文名称：
Recombinant Human Platelet-derived Growth Factor-BB
产品规格：
10μg
发货周期：
1～3天
产品价格：
询价
General Description
Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s.It is a disulfide-linked dimer consisting of two peptides-chain A andchain B.PDGF has three subforms:PDGF-AA,PDGF-BB,PDGF-AB.It is involved in a number of biological processes,including hyperplasia,embryonic neuron development,chemotaxis,and respiratory tubule epithelial cell development.The function of PDGF is mediated by two receptors(PDGFR-α and PDGFR-β).
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.
Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Molecular Weight
Approximately 24.8kDa,a disulfide-linked homodimeric protein containing two 110 amino acid residues polypeptide (B chain).
Purity
> 97% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3ng/mL,corresponding to a specific activity of> 3.3×105 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS,pH7.4.
Endotoxin
Less than 1 EU/μg of rHuPDGF-BB as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
AA Sequence
MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP
VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
相关搜索：重组人PDGF-BB，PDGF-BB, Human
Platelet-derived growth factor (PDGF) presenting in serum but absent from plasma was first discovered in animal study by Lynch and co-workers in the late 1980s.It is a disulfide-linked dimer consisting of two peptides-chain A andchain B.PDGF has three subforms:PDGF-AA,PDGF-BB,PDGF-AB.It is involved in a number of biological processes,including hyperplasia,embryonic neuron development,chemotaxis,and respiratory tubule epithelial cell development.The function of PDGF is mediated by two receptors(PDGFR-α and PDGFR-β).
Storage
This lyophilized preparation is stable at 2～8℃,but should be kept at -20℃ for long term storage,preferably desiccated.
Upon reconstitution,the preparation is stable for up to one week at 2～8℃.For maximal stability,apportion the reconstituted preparation into working aliquots and store at -20℃ to -70℃.Avoid repeated freeze/thaw cycles.
Molecular Weight
Approximately 24.8kDa,a disulfide-linked homodimeric protein containing two 110 amino acid residues polypeptide (B chain).
Purity
> 97% by SDS-PAGE and HPLC analyses.
Source
Escherichia coli.
Biological Activity
Fully biologically active when compared to standard.The ED50 as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3ng/mL,corresponding to a specific activity of> 3.3×105 IU/mg.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a 0.2μm filtered concentrated solution in PBS,pH7.4.
Endotoxin
Less than 1 EU/μg of rHuPDGF-BB as determined by LAL method.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom.Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1～1.0mg/mL.Stock solutions should be apportioned into working aliquots and stored at ≤ -20℃.
Further dilutions should be made in appropriate buffered solutions.
AA Sequence
MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP
VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
相关搜索：重组人PDGF-BB，PDGF-BB, Human